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Science and the Scientific: Growing Scientific Temper among Indians

Start Date :
Sep 16, 2020
Last Date :
Dec 31, 2020
23:45 PM IST (GMT +5.30 Hrs)
India Science (www.indiascience.in) is an Internet-based science Over-The-Top (OTT) TV channel. It is an initiative of the Department of Science and Technology (DST), Govt of India ...
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manjula
5 years 9 months ago
Science is different from scientific. Everybody know the science. but they do not follow the scientific method to work properly. Every year we produce lots of doctors and engineers they are studying science by reading her course material and other things, but they don't know how it is represent in our traditional and tradition and heritage. We had more number of engineers but poor number of dams and techniques. We are not beat for our ancient fore fathers. Tanjore temple, Kallani, Mullai p
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Arjun Sharma
5 years 9 months ago
Positive and negative controls should be specified to confirm/refute specific virus detection
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Arjun Sharma
5 years 9 months ago
Molecular biological validations; amplified PCR products must be validated either by running the products in a gel with a DNA ruler, or by direct DNA sequencing
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Arjun Sharma
5 years 9 months ago
The number of amplification cycles (less than 35; preferably 25-30 cycles);
In case of virus detection, >35 cycles only detects signals which do not correlate with infectious virus as determined by isolation in cell culture [reviewed in 2]; if someone is tested by PCR as positive when a threshold of 35 cycles or higher is used (as is the case in most laboratories in Europe & the US), the probability that said person is actually infected is less than 3%, the probability that said result is a fal
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Arjun Sharma
5 years 9 months ago
The temperature at which all reactions take place:
a) DNA melting temperature (>92°)
b) DNA amplification temperature (TaqPol specific)
c) Tm; the annealing temperature (the temperature at which the primers and probes reach the target binding/detachment, not to exceed 2 ̊C per primer pair). Tm heavily depends on GC content of the primers
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Arjun Sharma
5 years 9 months ago
. The primers and probes:
a) the concentration of primers and probes must be of optimal range
(100-200 nM)
b) must be specific to the target-gene you want to amplify
c) must have an optimal percentage of GC content relative to the total nitrogenous bases (minimum 40%, maximum 60%)
d) for virus diagnostics at least 3 primer pairs must detect 3 viral genes (preferably as far apart as possible in the viral genome
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Arjun Sharma
5 years 9 months ago
What is important when designing an RT-PCR Test and the quantitative RT-qPCR test described in the Corman-Drosten publication?
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Gopal Kota
5 years 9 months ago
DEAR ALL FELLOW CITIZENS,
The good news is VACCINE arriving within weeks and the must precaution after VACCINE is MASK to be considered.
Details attached please.
The fact is hard but the protection with VACCINE is for 9 months.
Dreams OF MASK less life is not yet becoming true.
Better get acquainted with MASK LIFE
The future generations may BORN with NATURAL MASK like KARNA born with GOLDEN SHEILD ?
In TV news we can watch many political leaders and celebrities without Mask, risky life
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Pallavi Singh
5 years 9 months ago
Science is very important for us.the role of science in our life is very huge.science only not a subject it's a part of life.its open ended experiments promote the creative and critical thinking of students.students thinks different. Science is important not for students, science is important to all.
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AYUSH SHARMA
5 years 9 months ago
Science is the study of the nature and behaviour of natural things and the knowledge that we obtain about them. ... A science is a particular branch of science such as physics, chemistry, or biology. Physics is the best example of a science which has developed strong, abstract theories.
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